I fondly remember my times cloning vectors… Not. My favourite was a multistep cloning and digesting protocol that I meticulously planned. It obviously failed numerous times. I got it right on the first try just making blunt ends and hoping it goes in correctly.
Ah, you cloned the wrong resistance gene again…
I fondly remember my times cloning vectors… Not. My favourite was a multistep cloning and digesting protocol that I meticulously planned. It obviously failed numerous times. I got it right on the first try just making blunt ends and hoping it goes in correctly.
Does it bother you when someone follows your protocol and it works on the first try?